17beta-Hydroxy-C19-steroid dehydrogenases from male guinea pig liver. Purification and characterization.
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چکیده
منابع مشابه
Some Properties of the Pyridine Nucleotide-specific 17@-Hydroxy Steroid Dehydrogenases of Guinea Pig Liver*?
The liver of the guinea pig was shown (1, 2) to contain two enzymes which catalyze the dehydrogenation of testosterone. One is located in the particulate fraction and specifically requires diphosphopyridine nucleotide as hydrogen acceptor; the other, present in the fraction of guinea pig homogenate not sedimented by 24,500 X g for 60 minutes, specifically requires triphosphopyridine nucleotide ...
متن کاملSome properties of the pyridine nucleotide-specific 17 beta-hydroxy steroid dehydrogenases of guinea pig liver.
The liver of the guinea pig was shown (1, 2) to contain two enzymes which catalyze the dehydrogenation of testosterone. One is located in the particulate fraction and specifically requires diphosphopyridine nucleotide as hydrogen acceptor; the other, present in the fraction of guinea pig homogenate not sedimented by 24,500 X g for 60 minutes, specifically requires triphosphopyridine nucleotide ...
متن کاملBile acid: CoASH ligases from guinea pig and porcine liver microsomes. Purification and characterization.
A procedure for the purification of the enzyme bile acid:CoA ligase from guinea pig liver microsomes was developed. Activity toward chenodeoxycholate, cholate, deoxycholate, and lithocholate co-purified suggesting that a single enzyme form catalyzes the activation of all four bile acids. Activity toward lithocholate could not be accurately assayed during the earlier stages of purification due t...
متن کاملPurification and characterization of monolysocardiolipin acyltransferase from pig liver mitochondria.
In mammalian tissues cardiolipin is rapidly remodeled by monolysocardiolipin acyltransferase subsequent to its de novo biosynthesis (Ma, B. J., Taylor, W. A, Dolinsky, V. W., and Hatch, G. M. (1999) J. Lipid Res. 40, 1837-1845). We report here the purification and characterization of a monolysocardiolipin acyltransferase activity from pig liver mitochondria. Monolysocardiolipin acyltransferase ...
متن کامل(NADP+) from Guinea-Pig Liver
As a result of studies of guinea-pig liver testosterone 17/J-dehydrogenase (NADP+) (EC 1.1.1.64), anew testosterone 17f8-dehydrogenase was discovered. The new enzyme was purified to a single homogeneous protein from the 105000g-supernatant fraction of guinea-pig liver by (NH4)2SO4 fractional precipitation and two gel-filtration stages, DEAE-cellulose column chromatography and hydroxyapatite col...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34848-2